Immunoassays for Pre-clinical and Clinical research
Luminous Consulting can prepare custom assay and workflow plans that will alleviate the development time required for researchers doing cell-based assays. Validation on-site or off-site is possible, depending on the project specifications. We can also offer solutions to make these assays more affordable and convenient to use in most cases that what might be currently off-the-shelf commercially.
Services include:
Design, Optimize and Validate multicolor Flow Cytometry assays (up to 30 parameters depending on the instrument)
Standardization of flow cytometers and multicolor panels across instruments and work sites.
Microscopy and fluorescent pathology for pre-clinical research
Bead-based analyte detection.
Cell Health, proliferation, apoptosis and specific biosensor assays like Calcium, pH, membrane potential, oxidative species detection.
Cytometer Experience:
Amnis Imagestream
Beckman Coulter
BD Biosciences
Cytek™ Aurora/ Northern Lights
Thermo Fisher instruments
However, cytometers are similar in theory and operation and all platforms can be used for our assay development services. This graphic demonstrates cell health applications from Sigma on the Amnis® ImageStream.
For microscopy, we can offer assay support, particularly in multicolor applications, amplification methods and high content screening assays. However, instrumentation and imaging is the responsibility of the client.
Here is a link to a presentation Kelly Lundsten gave on a 26-color assay for B-cell maturation optimized for the Cytek™ Aurora from 2019 and a multicolor flow talk on detecting T-cell chemokine and cytokine receptors and signaling: